Beta-Lactam Strip Test

Beta-Lactam Strip Test
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Product Details

Product Description
Kit Contents Amount Storage
Gold reaction wells 6 x 16 (96 total) 4°C
Test Strips 6 x 16 strips (96 total) 4°C
Positive Control (4 ppb Penicillin G) 1 vial 4°C
Negative Control 1 vial 4°C
Pipette tips 100 tips RT
REAGEN™ Beta-lactam Strip Test is a qualitative and rapid lateral flow assay designed to detect beta-lactam antibiotic residues in raw commingled cow’s milk. This state-of-the-art test uses a novel beta-lactam binding protein which eliminates the need for a heating block. This test is designed for point-of-care use or reference lab settings.The unique features of the kit are:
? Rapid strip test method – 7 minutes
? Novel binding protein that requires no heating step
? High sensitivity
? High reproducibility
Procedure Overview
The assay uses a competitive colloidal gold based format. The milk sample (200 ?L) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 min) to allow the beta-lactam binding protein on the gold particles to engage with any beta-lactam antibiotic present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to the beta-lactam antibiotic imprinted at the Test line (T-line), forming a signal (red line) at that position. If the beta-lactam binding protein on the gold particle has engaged with antibiotic present in the milk sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the T-line wh


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