Composition and preparation of Walne medium (modified from Laing, 1991).  Ferric chloride (FeCl3) Manganous chloride (MnCl2, 4H2O) Boric acid (H3BO3) EDTA(b), di-sodium salt Sodium di-hydrogen orthophosphate (NaH2PO4, 2H2O) Sodium nitrate (NaNO3) Zinc chloride (ZnCl2) Cobaltous chloride (CoCl2,6 H2O) Ammonium molybdate ((NH4)6Mo7O24, 4H2O) Cupric sulphate (CuSO4, 5H2O) Concentrated HCl Other Chemicals NH4SO4 Chloroform Phenol Sodium nitroprusside NaOH Sodium Citrate Chlorox Ethanol Iodine Crystals Potassium Iodide (KI) Methylene Blue Glasswares/ plasticwares Carbuoys Beakers Aerators Air hose Sinkers Fluorescent lamps Fluorescent tubes Pipettes Micropipettes Aspirator Reagent Bottles Dark reagent bottles Graduated Cylinder Test tubes Test tube racks Electric stove / gas stove Rubber stopper UV light Big pot Autoclave Erlenmeyer flasks  Basins (8L) Carbuoy culture shelf Haemacytometer Counter Pail Bunsen burner Fume hood  pH meter BOD bottles Spectrophotometer Centrifuge Dextrose bottles Air stones Muffle furnace Dessicator Experimental tanks Fiber glass tank (outdoor fiber tank)