Recombinant Carboxypeptidase B?with His Tag?
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Supplier Details
Zhuhai Gene-Biocon Biological Technology Co., Ltd.
Free Member (2023)
[ Zhuhai, GU,China ]
Business Type: Manufacturer
Supplier has 5 products
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Product Description
CAS: 9025-24-5
Recombinant Carboxypeptidase B ?with His Tag? is a lyophilized powder that is isolated and purified from recombinant Pichia pastoris and then freeze-dried, and has the same enzyme activity and specificity as natural carboxypeptidase a and b.
Recombinant Carboxypeptidase B ?with His Tag?Specifications
Product Name Recombinant Lysyl Endopeptidase Biochemical Grade
Appearances White or off-white powder
Specific Activity ?80U/mg pro
MW 36kD±3.6kD
Purity ?95%?SDS-PAGE?
Protein Content 20%-60%
Residue DNA ?100ng/mg pro
Residue HCP ?100ng/mg pro
Optimum pH 7.09.0
Solubility Soluble in buffer
Storage Conditions -15°C below, protected from light?sealed.
Carboxypeptidase B Application
Production of various proteins or polypeptides, such as insulin and analogs, cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like.
Quality testing of antibodies.
Determination of the C-terminal amino acid of a protein
Carboxypeptidase B Recommended Method
Protein preparation: The protein was dissolved in a solution of 25 mM Tris-HCl, pH 7.6.
Enzyme preparation: The desired enzyme powder was weighed and dissolved in 25 mM Tris-HCl pH 7.6 to give an enzyme solution concentration of 110 mg/ml.
Reaction recommended enzyme amount: target protein mass ratio 1:50 1:1000, optimum pH is 7.09.0.
Recombinant Carboxypeptidase-B Transport and Storage Stability
Transportation stability: It is transported in an ice pack to keep it active.
Storage stability: The lyophilized powder can be stored for at least 24 months at -15°C below.
25 mM Tris-HCl pH 7.6 was dissolved and stored at -15°C below, and repeatedly frozen and thawed 10 times without loss of activity.
It is recommended not to exceed 7 days after storage at 28°C, and no more than 30 days for -15°C below.
Notice and Disclaimer
Activity is competitively inhibited by arginine and lysine, and metal ion chelating agents such as EDTA also inhibit enzyme activity.
The product is hygroscopic and should be taken after equilibration at room temperature.
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
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About Us
Gene-Biocon is a manufacture of high-quality pharmaceutical and cosmetic raw supplies. Gene-Biocon offers Insulin Degludec, Insulin Aspart, Liraglutide, Semaglutide, Recombinant Trypsin, Recombinant Lysyl Endopeptidase, Cell growth factors and Cosmetic materials. In addition to normal products, Gene-Biocon offers supreme customized products with OEM-equivalent quality to meet customers' needs.
