BlueGene Biotech Canine Caspase 9 ELISA kit

E08C0096 Canine Caspase 9 ELISA kit



Canine Caspase 9 ELISA kit is suitable for the detection of samples from Canine species. Caspase 9 can also be called ICE-LAP6, MCH6, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, Caspase-9L, ICE-LAP6, Mch6, CASP-9, PPP1R56, CASP9.



Specifications of Canine Caspase 9 ELISA kit

Product Information

Cat. No. E08C0096

Product Name Canine Caspase 9 ELISA kit

Species Canine

Product Size 48 Tests / 96 Tests

Concentration 10-25 ng/mL

Sensitivity 0.1 ng/mL

Principal Competitive ELISA

Sample Volume 100 ul

Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time 90 minutes

Platform Microplate Reader

Conjugate HRP

Detection Method Colorimetric

Storage 2-8°C



Kit Components

MATERIALS SPECIFICATION QUANTITY

MICROTITER PLATE 96 wells stripwell

ENZYME CONJUGATE 6.0 mL 1 vial

STANDARD A 0.5mL 0 ng/mL 1 vial

STANDARD B 0.5mL 0.5 ng/mL 1 vial

STANDARD C 0.5mL 1 ng/mL 1 vial

STANDARD D 0.5mL 2.5 ng/mL 1 vial

STANDARD E 0.5mL 5 ng/mL 1 vial

STANDARD F 0.5mL 10 ng/mL 1 vial

SUBSTRATE A 6 mL 1 vial

SUBSTRATE B 6 mL 1 vial

STOP SOLUTION 6 mL 1 vial

WASH SOLUTION 100 x 10 mL 1 vial

BALANCE SOLUTION 3 mL 1 vial



Principle of the Assay



CASP9 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CASP9 antibody and an CASP9-HRP conjugate. The assay sample and buffer are incubated together with CASP9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP9 concentration since CASP9 from samples and CASP9-HRP conjugate compete for the anti-CASP9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP9 from the sample, fewer sites are left to bind CASP9-HRP conjugate. A standard curve is plotted relating the intensity of the color O.D. to the concentration of standards. The CASP9 concentration in each sample is interpolated from this standard curve.



Quality Control on Canine Caspase 9 Human Insulin ELISA kit

Coefficient of Variance Intra Variation% ?10%

Inter Variation% ?12%

Recovery 85-103%

Linearity Diluent ratio Range %

1:02 86-105

1:04 83-109

1:08 82-108

Specificity/Cross-reactivity No significant cross-reactivity or interference between APAF3 and analogues was observed.



Elisa kit supplier Shanghai BlueGene Biotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay ELISA kits and registered trademark cgbio® ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit. We offer thousands of ELISA kits for researchers worldwide since 2008. As one elisa kit manufacturer, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGene Biotech ELISA kits.