BlueGene Biotech Canine Collagen Type II Alpha 1 ELISA kit

E08C0323 Canine Collagen Type II Alpha 1 ELISA kit



Canine Collagen Type II Alpha 1 ELISA kit is suitable for the detection of samples from Canine species. Collagen Type II Alpha 1 can also be called COL2-A1, COL2A1, COL2 A1, SEDC, Chondrocalcin, COL2?1, COL2-?1, COL2 ?1.



Specifications of Canine Collagen Type II Alpha 1 ELISA kit

Product Information

Cat. No. E08C0323

Product Name Canine Collagen Type II Alpha 1 ELISA kit

Species Canine

Product Size 48 Tests / 96 Tests

Concentration 10-250 ng/mL

Sensitivity 1.0 ng/mL

Principal Competitive ELISA

Sample Volume 100 ul

Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time 90 minutes

Platform Microplate Reader

Conjugate HRP

Detection Method Colorimetric

Storage 2-8°C



Kit Components

MATERIALS SPECIFICATION QUANTITY

MICROTITER PLATE 96 wells stripwell

ENZYME CONJUGATE 6.0 mL 1 vial

STANDARD A 0.5mL 0 ng/mL 1 vial

STANDARD B 0.5mL 10 ng/mL 1 vial

STANDARD C 0.5mL 25 ng/mL 1 vial

STANDARD D 0.5mL 50 ng/mL 1 vial

STANDARD E 0.5mL 100 ng/mL 1 vial

STANDARD F 0.5mL 250 ng/mL 1 vial

SUBSTRATE A 6 mL 1 vial

SUBSTRATE B 6 mL 1 vial

STOP SOLUTION 6 mL 1 vial

WASH SOLUTION 100 x 10 mL 1 vial

BALANCE SOLUTION 3 mL 1 vial



Principle of the Assay



COL2a1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL2a1 antibody and an COL2a1-HRP conjugate. The assay sample and buffer are incubated together with COL2a1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL2a1 concentration since COL2a1 from samples and COL2a1-HRP conjugate compete for the anti-COL2a1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL2a1 from the sample, fewer sites are left to bind COL2a1-HRP conjugate. A standard curve is plotted relating the intensity of the color O.D. to the concentration of standards. The COL2a1 concentration in each sample is interpolated from this standard curve.



Quality Control on Canine Collagen Type II Alpha 1 ELISA kit

Coefficient of Variance Intra Variation% ?10%

Inter Variation% ?12%

Recovery 87-104%

Linearity Diluent Ratio Range %

1:02 87-105

1:04 84-109

1:08 81-108

Specificity/Cross-reactivity No significant cross-reactivity or interference between COL2a1 and analogues was observed.



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